psip1 antibody Search Results


ledgf  (Proteintech)
92
Proteintech ledgf
A , Representative ChIP-seq tracks of H3K36me2, <t>LEDGF,</t> <t>and</t> <t>HDGF2</t> chromatin co-occupancy in H3-WT (DIPG10 and pcGBM2) and H3K27M (DIPG4, DIPG6, and DIPG13) cells. B , Violin plots showing enrichment of H3K36me2, LEDGF, or HDGF2, respectively, for genes categorized as low, mid, or high based on their mRNA expression. The central thick dash line indicates the mean value of each plot; the upper thin dash line indicates the top 25% percentile and the lower one for the bottom 25% percentile. C , Metaprofile plots of H3K27me3, H3K36me2, LEDGF, and HDGF2 ChIP-seq data in HEK293T cells ectopically expressing wildtype histone H3 (H3-WT) or H3K27M for 24 hrs. Genes exhibiting loss of H3K27me3 by H3K27M were categorized by its loss upstream or downstream from transcription start sites (TSS) and subsequent changes in H3K36me2, LEDGF, and HDGF2 occupancies were presented below. Data were presented within a 500-kb window upstream or downstream from TSS. D , Top, metaprofile plots of LEDGF and HDGF2 occupancy in wildtype or NSD2-KO DIPG13 cells (clone#7 and clone#10). Bottom, representative ChIP-seq tracks for the top panel. Overlayed panels were presented at the bottom to better illustrate the differences. ****p<0.0001 by Student’s t-test.
Ledgf, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ledgf/product/Proteintech
Average 92 stars, based on 1 article reviews
ledgf - by Bioz Stars, 2026-02
92/100 stars
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psip1 novus  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology psip1 novus
Isoform switch from PBX1a and PBX1b during hESC differentiation. a Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. b The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. c The sequence difference of three protein isoforms of PBX1 and the main functional domains. d The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. e The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. f The expression levels of <t>PSIP1</t> and SRSF1 show significant positive correlations with the expression level of PBX1a. Also see Additional file : Figures S9, S10
Psip1 Novus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psip1 novus/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
psip1 novus - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
anti-n-psip1 antibody  (Carl Roth GmbH)
90
Carl Roth GmbH anti-n-psip1 antibody
Isoform switch from PBX1a and PBX1b during hESC differentiation. a Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. b The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. c The sequence difference of three protein isoforms of PBX1 and the main functional domains. d The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. e The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. f The expression levels of <t>PSIP1</t> and SRSF1 show significant positive correlations with the expression level of PBX1a. Also see Additional file : Figures S9, S10
Anti N Psip1 Antibody, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-n-psip1 antibody/product/Carl Roth GmbH
Average 90 stars, based on 1 article reviews
anti-n-psip1 antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A , Representative ChIP-seq tracks of H3K36me2, LEDGF, and HDGF2 chromatin co-occupancy in H3-WT (DIPG10 and pcGBM2) and H3K27M (DIPG4, DIPG6, and DIPG13) cells. B , Violin plots showing enrichment of H3K36me2, LEDGF, or HDGF2, respectively, for genes categorized as low, mid, or high based on their mRNA expression. The central thick dash line indicates the mean value of each plot; the upper thin dash line indicates the top 25% percentile and the lower one for the bottom 25% percentile. C , Metaprofile plots of H3K27me3, H3K36me2, LEDGF, and HDGF2 ChIP-seq data in HEK293T cells ectopically expressing wildtype histone H3 (H3-WT) or H3K27M for 24 hrs. Genes exhibiting loss of H3K27me3 by H3K27M were categorized by its loss upstream or downstream from transcription start sites (TSS) and subsequent changes in H3K36me2, LEDGF, and HDGF2 occupancies were presented below. Data were presented within a 500-kb window upstream or downstream from TSS. D , Top, metaprofile plots of LEDGF and HDGF2 occupancy in wildtype or NSD2-KO DIPG13 cells (clone#7 and clone#10). Bottom, representative ChIP-seq tracks for the top panel. Overlayed panels were presented at the bottom to better illustrate the differences. ****p<0.0001 by Student’s t-test.

Journal: bioRxiv

Article Title: The H3K36me2 writer-reader dependency in H3K27M-DIPG

doi: 10.1101/2021.01.06.425580

Figure Lengend Snippet: A , Representative ChIP-seq tracks of H3K36me2, LEDGF, and HDGF2 chromatin co-occupancy in H3-WT (DIPG10 and pcGBM2) and H3K27M (DIPG4, DIPG6, and DIPG13) cells. B , Violin plots showing enrichment of H3K36me2, LEDGF, or HDGF2, respectively, for genes categorized as low, mid, or high based on their mRNA expression. The central thick dash line indicates the mean value of each plot; the upper thin dash line indicates the top 25% percentile and the lower one for the bottom 25% percentile. C , Metaprofile plots of H3K27me3, H3K36me2, LEDGF, and HDGF2 ChIP-seq data in HEK293T cells ectopically expressing wildtype histone H3 (H3-WT) or H3K27M for 24 hrs. Genes exhibiting loss of H3K27me3 by H3K27M were categorized by its loss upstream or downstream from transcription start sites (TSS) and subsequent changes in H3K36me2, LEDGF, and HDGF2 occupancies were presented below. Data were presented within a 500-kb window upstream or downstream from TSS. D , Top, metaprofile plots of LEDGF and HDGF2 occupancy in wildtype or NSD2-KO DIPG13 cells (clone#7 and clone#10). Bottom, representative ChIP-seq tracks for the top panel. Overlayed panels were presented at the bottom to better illustrate the differences. ****p<0.0001 by Student’s t-test.

Article Snippet: The antibodies used in this study are listed below: LEDGF (Proteintech) Rabbit Polyclonal, Cat # 25504-1-AP HDGF2 (Proteintech) Rabbit Polyclonal, Cat # 15134-1-AP H3K27me3 (Cell Signaling) Rabbit Monoclonal C36B11, Cat # 9733 H3K36me2 (Cell Signaling) Rabbit Monoclonal C75H12, Cat # 2901 H3K36me3 (Cell Signaling) Rabbit Monoclonal D5A7, Cat # 4909 NSD1 (UC Davis/NIH NeuroMab Facility) Mouse Monoclonal,N312/10 NSD2 (Millipore) Mouse Monoclonal 29D1, Cat# MABE191 NSD3 (Cell Signaling) Rabbit Monoclonal D4N9N, Cat # 92056 ASH1L (Bethyl Laboratories) Rabbit Polyclonal, Cat # A301-748A SETD2 (Bio-Rad) Mouse Monoclonal OTI1E1, Cat # VMA00449 GAPDH (Cell Signaling) Rabbit Monoclonal D16H11, Cat # 5174 Histone H3 (Abcam) Rabbit Monoclonal EPR16987, Cat # ab176842 Anti-Flag (Sigma) Mouse Monoclonal M2, Cat # F1804 H2Av (Active Motif) Rabbit Polyclonal, Cat # 39715

Techniques: ChIP-sequencing, Expressing

A , Top, a schematic illustration of the design of Cell Penetrating Peptide (CPP). A HIV-based cell entry peptide was linked to a H3K36me2 peptide (histone H3 21-43 a.a., Cargo Peptide) by a disulfide bond. Bottom, Amino acid (a.a.) sequence of H3K36me2 linked CPP. B . A CellTiter-Glo® cell survival assay for H3-WT (DIPG10 and pcGBM2) and H3K27M (DIPG6 and DIPG13) cells treated with control (vehicle only) or H3K36me2-CPP. Cells were assayed at 72 hours after dosing with a titration of control or H3K36me2-CPP and data were presented by ratios of CellTiter-Glo signals in control versus H3K36me2-CPP treated cells. C , A metaprofile of ChIP-seq analysis for changes in LEDGF and HDGF2 occupancy of genes decorated by H3K36me2 and H3K36me3 in DIPG13 cells treated with a control vehicle (Red) or a H3K36me2-CPP (Blue).

Journal: bioRxiv

Article Title: The H3K36me2 writer-reader dependency in H3K27M-DIPG

doi: 10.1101/2021.01.06.425580

Figure Lengend Snippet: A , Top, a schematic illustration of the design of Cell Penetrating Peptide (CPP). A HIV-based cell entry peptide was linked to a H3K36me2 peptide (histone H3 21-43 a.a., Cargo Peptide) by a disulfide bond. Bottom, Amino acid (a.a.) sequence of H3K36me2 linked CPP. B . A CellTiter-Glo® cell survival assay for H3-WT (DIPG10 and pcGBM2) and H3K27M (DIPG6 and DIPG13) cells treated with control (vehicle only) or H3K36me2-CPP. Cells were assayed at 72 hours after dosing with a titration of control or H3K36me2-CPP and data were presented by ratios of CellTiter-Glo signals in control versus H3K36me2-CPP treated cells. C , A metaprofile of ChIP-seq analysis for changes in LEDGF and HDGF2 occupancy of genes decorated by H3K36me2 and H3K36me3 in DIPG13 cells treated with a control vehicle (Red) or a H3K36me2-CPP (Blue).

Article Snippet: The antibodies used in this study are listed below: LEDGF (Proteintech) Rabbit Polyclonal, Cat # 25504-1-AP HDGF2 (Proteintech) Rabbit Polyclonal, Cat # 15134-1-AP H3K27me3 (Cell Signaling) Rabbit Monoclonal C36B11, Cat # 9733 H3K36me2 (Cell Signaling) Rabbit Monoclonal C75H12, Cat # 2901 H3K36me3 (Cell Signaling) Rabbit Monoclonal D5A7, Cat # 4909 NSD1 (UC Davis/NIH NeuroMab Facility) Mouse Monoclonal,N312/10 NSD2 (Millipore) Mouse Monoclonal 29D1, Cat# MABE191 NSD3 (Cell Signaling) Rabbit Monoclonal D4N9N, Cat # 92056 ASH1L (Bethyl Laboratories) Rabbit Polyclonal, Cat # A301-748A SETD2 (Bio-Rad) Mouse Monoclonal OTI1E1, Cat # VMA00449 GAPDH (Cell Signaling) Rabbit Monoclonal D16H11, Cat # 5174 Histone H3 (Abcam) Rabbit Monoclonal EPR16987, Cat # ab176842 Anti-Flag (Sigma) Mouse Monoclonal M2, Cat # F1804 H2Av (Active Motif) Rabbit Polyclonal, Cat # 39715

Techniques: Sequencing, Clonogenic Cell Survival Assay, Control, Titration, ChIP-sequencing

Isoform switch from PBX1a and PBX1b during hESC differentiation. a Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. b The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. c The sequence difference of three protein isoforms of PBX1 and the main functional domains. d The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. e The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. f The expression levels of PSIP1 and SRSF1 show significant positive correlations with the expression level of PBX1a. Also see Additional file : Figures S9, S10

Journal: Genome Biology

Article Title: Alternative splicing links histone modifications to stem cell fate decision

doi: 10.1186/s13059-018-1512-3

Figure Lengend Snippet: Isoform switch from PBX1a and PBX1b during hESC differentiation. a Genome browser view shows the AS event and H3K36me3 signals of PBX1 upon hESC differentiation. The green horizontal bars below the ChIP-seq tracks indicate the narrow peaks called by MACS2. b The inclusion level for exon 7 of PBX1 is significantly correlated to the H3K36me3 signals over this exon across cell lineages. c The sequence difference of three protein isoforms of PBX1 and the main functional domains. d The relative expressions of PBX1a and PBX1b in 56 cells/tissues, representing the differential expressions of two isoforms in three groups based on their developmental states. e The expression levels of NANOG and OCT4 genes are negatively correlated with the expression of PBX1b. f The expression levels of PSIP1 and SRSF1 show significant positive correlations with the expression level of PBX1a. Also see Additional file : Figures S9, S10

Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and PBX1b (Santa Cruz).

Techniques: ChIP-sequencing, Sequencing, Functional Assay, Expressing

Isoform switch of PBX1 links H3K36me3 to hESC fate decision. a qRT-PCR and western blot show the expression levels of Yamanaka factors in H1, MSC, and IMR90 cells. Whiskers denote the standard deviations of three replicates. b RT-PCR and western blot show the isoform switches between PBX1a and PBX1b from H1 cells to differentiated cells. c i. ChIP-PCR shows the differential binding of PBX1b to NANOG promoter in H1 cells and differentiated cells; ii. ChIP-PCR shows the reduced H3K36me3 signal in differentiated cells; iii. ChIP-PCR shows the differential recruitment of PSIP1 to exon 7 of PBX1. d RIP-PCR show the differential recruitment of SRSF1 around exon 7 of PBX1. e Co-IP shows the overall physical interaction between PSIP1 and SRSF1 in all studied cell types. f The mechanism by which H3K36me3 is linked to cell fate decision by regulating the isoform switch of PBX1, which functions upstream of the pluripotency regulatory network. Also see Additional file : Figures S9, S10

Journal: Genome Biology

Article Title: Alternative splicing links histone modifications to stem cell fate decision

doi: 10.1186/s13059-018-1512-3

Figure Lengend Snippet: Isoform switch of PBX1 links H3K36me3 to hESC fate decision. a qRT-PCR and western blot show the expression levels of Yamanaka factors in H1, MSC, and IMR90 cells. Whiskers denote the standard deviations of three replicates. b RT-PCR and western blot show the isoform switches between PBX1a and PBX1b from H1 cells to differentiated cells. c i. ChIP-PCR shows the differential binding of PBX1b to NANOG promoter in H1 cells and differentiated cells; ii. ChIP-PCR shows the reduced H3K36me3 signal in differentiated cells; iii. ChIP-PCR shows the differential recruitment of PSIP1 to exon 7 of PBX1. d RIP-PCR show the differential recruitment of SRSF1 around exon 7 of PBX1. e Co-IP shows the overall physical interaction between PSIP1 and SRSF1 in all studied cell types. f The mechanism by which H3K36me3 is linked to cell fate decision by regulating the isoform switch of PBX1, which functions upstream of the pluripotency regulatory network. Also see Additional file : Figures S9, S10

Article Snippet: The protein–DNA complex was precipitated with ChIP-Grade Protein G Magnetic Beads (Cell Signalling) and ChIP-validated antibodies against H3K36me3 (Abcam), PSIP1 (Novus), and PBX1b (Santa Cruz).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Co-Immunoprecipitation Assay